Hypothermic Oxygenated machine PErfusion, better organ quality saves lifes


  1. Schlegel A, Kron P, Graf R, Dutkowski P and Clavien PA., Warm vs. cold perfusion technique to rescue rodent liver grafts. J Hepatol. 2014 Jul 30. pii: S0168-8278(14)00522-4. doi: 10.1016/j.jhep.2014.07.023. (in press)
  2. Dutkowski P*, Schlegel A*, De Oliveira M, Müllhaupt B, Neff F, Clavien P.-A. HOPE for human liver grafts obtained after cardiac arrest J Hepatol. 2014 Apr;60(4):765-72
  3. Schlegel A, Kron P, Graf R, Clavien P.-A., Dutkowski P. Hypothermic oxygenated machine perfusion (HOPE) down-regulates the immune response in a rat model of liver transplantation Ann Surg 2014 in press
  4. Schlegel A, Graf R, Clavien P.-A, Dutkowski P. Hypothermic oxygenated machine perfusion protects from biliary injury in a rodent model of liver transplantation after cardiac arrest. J Hepatol. 2013 Nov;59(5):984-91
  5. Schlegel A, de Rougemont O, Graft R, Clavien PA, Dutkowski P. Protective mechanism of end-ischemic cold machine perfusion in DCD liver grafts, Journal of Hepatology 2013 vol. 58 j 278–286
  6. De Rougemont O, Dutkowski P, Clavien PA. Biological modulation of liver ischemia-reperfusion injury. Current opinion in Organ Transplantation 2010, 15:183-189.
  7. De Rougemont O, Graf R, Clavien PA, Dutkowski P. One hour hypothermic oxygenated perfusion rescues non viable liver allografts donated after cardiac death, Ann Surg 2009, 250: 674-683.
  8. Dutkowski P, De Rougemont O, Müllhaupt B, Clavien PA. Current and future trends in liver transplantation in Europe. Gastroenterology. 2010 Mar;138(3):802-9.e1-4.

  9. Duenschede F, Tyble E, Kiemer AK, Dutkowski P, Erbes K, Kircher A, Gockel I, Zechner U, Schad A, Lang H, Junginger T, Kempski O. Bcl-2 upregulation after 3-nitropropionic acid preconditioning in warm rat liver ischemia, Shock 2008, 30: 699-704.
  10. Dutkowski P, de Rougemont O. Clavien P-A. Machine perfusion for « marginal » liver grafts. Am J Transpl 2008, 8 : 917-924
  11. Duenschede F, Westermann S, Miesner I, Albrecht-Schock S, Kneist W, Korenkov M, Schad A, Dutkowski P, Kiemer AL, Junginger T. Hepatocellular Injury of Nonischemic Liver Tissue after Selective Clamping in Rats - Protective Action by Pharmacological Pretreatment with Lipoic Acid. Eur Surg Res. 2007 Jul, 39(6):325-331.
  12. Duenschede F, Erbes K, Kircher A, Westermann S, Schad A, Riegler N, Ewald P, Dutkowski P, Kiemer K, Kempski O, Junginger T. Protection from hepatic ischemia/reperfusion injury and improvement of liver regeneration by a-lipoic acid. Shock 2007 Jun,27(6): 644-651.
  13. Dutkowski P, Furrer K, Tian Y, Graf R, Clavien P-A. Novel short term hypothermic oxygenated perfusion (HOPE) system prevents injury in rat liver graft from non-heart beating donor. Ann Surg 2006 Dec,244(6): 968-976.
  14. Duenschede F, Westermann S, Riegler N, Ewald P, Kircher A, Schaefer H, Schneider J, Schad A, Dutkowski P, Junginger T. Different protection mechanisms after pre-treatment with glycine or alpha-lipoic acid in a rat model of warm hepatic ischemia. Eur Surg Res 2006, 38(6): 503-512.
  15. Dutkowski P, Graf R, Clavien P-A. Rescue of the cold preserved rat liver by hypothermic oxygenated machine perfusion. Am J Transplant. 2006 May;6(5):903-12.
  16. Dutkowski P, Krug A, Krysiak M, Dunschede F, Seifer JK, Junginger T. Detection of mitochondrial electron chain carrier redox status by transhepatic light intensity during rat liver reperfusion. Cryobiology. 2003 Oct;47(2):125-42.
  17. Dutkowski P, Wahl W, Winkelbach V, Watzka M, Krysiak M, Junginger T. Calcium prevents loss of glutathione and reduces oxidative stress upon reperfusion in the perfused liver. Int J Surg Investig. 2000;2(1):1-7.
  18. Dutkowski P, Schonfeld S, Heinrich T, Watzka M, Winkelbach V, Krysiak M, Odermatt B, Junginger T. Reduced oxidative stress during acellular reperfusion of the rat liver after hypothermic oscillating perfusion. Transplantation. 1999 Jul 15;68(1):44-50.

  19. Dutkowski P, Odermatt B, Heinrich T, Schonfeld S, Watzka M, Winkelbach V, Krysiak M, Junginger T. Hypothermic oscillating liver perfusion stimulates ATP synthesis prior to transplantation. J Surg Res. 1998 Dec;80(2):365-72.

  20. Dutkowski P, Schonfeld S, Odermatt B, Heinrich T, Junginger T. Rat liver preservation by hypothermic oscillating liver perfusion compared to simple cold storage. Cryobiology. 1998 Feb;36(1):61-70.

  21. Dutkowski P, Heinrich T, Schonfeld S, Odermatt B, Junginger T. Stimulation of anaerobic glycolysis enhances liver cell viability during cold hypoxic incubation of liver slices. Transplant Proc. 1998 Feb;30(1):206-10.

  22. Dutkowski P, Southard JH, Junginger T. Liver metabolism during cold ischemic incubation in UW solution in the rat model] Langenbecks Arch Chir. 1997;382(6):343-8.



  • P. Dutkowski
  • A. Schlegel
  • P. Kron
  • M. de Oliveira
  • O. de Rougemont
  • K. Slankamenac
  • R. Graf
  • P.-A. Clavien


Department of Surgery and Transplantation

University Hospital Zurich

Rämistrasse 100, CH-8091 Zürich



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